Trefoil is a graphic form composed of three lobes.
Trefoil may also be:
- Clover, a widespread trifoliate plant family
- Trefoil Island, Tasmania, Australia
- Little Trefoil Island, Tasmania, Australia
- Trefoil knot, a kind of knot
- Certain species in Lotus (genus)
- A variation of the card game La Belle Lucie
- The Trefoil Guild for adult members of Scouts and Guides, see Scouting and Guiding in the United Kingdom or Scouting and Guiding in Australia
- A variety of Girl Scout cookie
- The flowering plant Lotus corniculatus commonly known as birdsfoot trefoil
- Higher-order aberration in optics
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